A07

A07: Summary

While the role of immune cells, oligodendrocytes and axons in multiple sclerosis (MS) is well studied, much less is known about the contribution of astrocytes to this neuroinflammatory disease. In the present project, we aim to elucidate the role of cAMP signaling in astrocyte function in healthy and in inflamed tissue. Therefore, new cAMP sensors as well as photoactivatable adenylyl cyclases with improved properties will be developed. With these tools we will study cAMP signaling in hippocampal astrocytes in response to neurotransmitters and neuronal activity, both in healthy animals and in animals with experimental autoimmune encephalomyelitis (EAE), an animal model of MS.

Dr Christine E. Gee

Selected publications

 

  1.  

  2. Perez-Alvarez A, Fearey BC, O'Toole RJ, Yang W, Arganda-Carreras I, Lamothe-Molina PJ, Moeyaert B, Mohr MA, Panzera LC, Schulze C, Schreiter ER, Wiegert JS, Gee CE, Hoppa MB, Oertner TG (2020) Freeze-frame imaging of synaptic activity using SynTagMA. Nat Commun  11:2464
  3. Scheib U, Broser M, Constantin OM, Yang S, Gao S, Mukherjee S, Stehfest K, Nagel G, Gee CE, Hegemann P (2018) Rhodopsin-cyclases for photocontrol of cGMP/cAMP and 2.3 Å structure of the adenylyl cyclase domain. Nat Commun 9:2046
  4. Bitzenhofer SH, Ahlbeck J, Wolff A, Wiegert JS, Gee CE, Oertner TG, Hanganu-Opatz IL (2017) Layer-specific optogenetic activation of pyramidal neurons causes beta-gamma entrainment of neonatal networks. Nat Commun8:14563. doi: 10.1038/ncomms14563.
  5. Scheib U*, Stehfest K*, Gee CE*, Körschen HG, Fudim R, Oertner TG, Hegemann P (2015) The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling. Sci Signal 8:rs8. doi: 10.1126/scisignal.aab0611. *equal contribution
  6. Gee CE, Peterlik D, Neuhäuser C, Bouhelal R, Kaupmann K, Laue G, Uschold-Schmidt N, Feuerbach D, Zimmermann K, Ofner S, Cryan JF, van der Putten H, Fendt M, Vranesic I, Glatthar R & Flor PJ. (2014) Blocking metabotropic glutamate receptor subtype 7 (mGlu7) via the venus flytrap domain (VFTD) inhibits amygdala plasticity, stress and anxiety-related behavior. J Biol Chem 289:1097510987.
  7. Hoyer D, Dürst T, Fendt M, Jacobson LH, Betschart C, Hintermann S, Behnke D, Cotesta S, Laue G, Ofner S, Legangneux E & Gee CE (2013) Distinct effects of IPSU and suvorexant on mouse sleep architecture. Frontiers in Neurosci 7:235.
  8. Fendt, M, Imobersteg S, Peterlik D, Chaperon F, Mattes C, Wittmann C, Olpe H-R, Mosbacher J, Vranesic I, van der Putten PH, McAllister KH, Flor PJ & Gee CE (2013) Differential roles of mGlu7 and mGlu8 in amygdala-dependent behavior and physiology. Neuropharmacology 72:215-233.
  9. Chaperon F, Fendt M, Kelly PH, Lingenhoehl K, Mosbacher J, Olpe HR, Schmid P, Sturchler C, McAllister KH, van der Putten PH & Gee CE (2012) Gastrin-releasing peptide signaling plays a limited and subtle role in amygdala physiology and aversive memory. PLoS ONE 7: e34963.
  10. Mang GM, Dürst T, Bürki H, Imobersteg S, Abramowski D, Schuepbach E, Hoyer D, Fendt M & Gee CE (2012) The dual orexin receptor antagonist almorexant induces sleep and decreases orexin induced locomotion by blocking orexin 2 receptors. SLEEP 35:1625-1635.
  11. Matsumoto-Miyai K, Sololowska E, Zurlinden A, Gee CE, Lüscher D, Hettwer S, Wölfel J, Ladner AP, Ster J, Gerber U, Rülicke T, Kunz B & P Sonderegger (2009) Coincident pre- and postsynaptic activation induces dendritic filopodia via neurotrypsin-dependent agrin cleavage. Cell 136:1161-1171.

Prof. Dr Christian Lohr

Selected publications

 

  1. Beiersdorfer A, Wolburg H, Grawe J, Scheller A, Kirchhoff F, Lohr C (2020) Sublamina-specific organization of the blood brain barrier in the mouse olfactory nerve layer. Glia 68:631-645
  2. Rotermund N, Winandy S, Fischer T, Schulz K, Fregin T, Alstedt N, Buchta M, Bartels J, Carlström M, Lohr C, Hirnet D (2018) Adenosine A1 receptor activates background potassium channels and modulates information processing in olfactory bulb mitral cells. J Physiol 596:717-733
  3. Droste D, Seifert G, Seddar L, Jädtke O, Steinhäuser C, Lohr C (2017) Ca2+-permeable AMPA receptors in mouse olfactory bulb astrocytes. Sci Rep. 7:44817.
  4. Stavermann M, Meuth P, Doengi M, Thyssen A, Deitmer JW, Lohr C (2015) Calcium-induced calcium release and gap junctions mediate large-scale calcium waves in olfactory ensheathing cells in situ. Cell Calcium 58:215-225. 3. Lohr C, Grosche A, Reichenbach A, Hirnet D (2014) Purinergic neuron-glia interactions in sensory systems. Pflugers Arch 466:1859–1872.
  5. Thyssen A, Stavermann M, Buddrus K, Doengi M, Ekberg JA, St John JA, Deitmer JW, Lohr C (2013) Spatial and developmental heterogeneity of calcium signaling in olfactory ensheathing cells. GLIA 61:327-337.
  6. Stavermann M, Buddrus K, St John JA, Ekberg JA, Nilius B, Deitmer JW, Lohr C (2012) Temperature-dependent calcium-induced calcium release via InsP3 receptors in mouse olfactory ensheathing glial cells. Cell Calcium52:113-123.
  7. Thyssen A, Hirnet D, Wolburg H, Schmalzing G, Deitmer JW, Lohr C (2010) Ectopic vesicular neurotransmitter release along sensory axons mediates neurovascular coupling via glial calcium signaling. Proc Natl Acad Sci USA107:15258-15263.
  8. Doengi M, Hirnet D, Coulon P, Pape HC, Deitmer JW, Lohr C (2009) GABA uptake-dependent Ca2+ signaling in olfactory bulb astrocytes. Proc Natl Acad Sci USA 106:17570-17575.
  9. Doengi M, Deitmer JW, Lohr C (2008) New evidence for purinergic signaling in the olfactory bulb: A2A and P2Y1 receptors mediate intracellular calcium release in astrocytes. FASEB J 22:2368-2378.

Our Team

Prof. Dr Christian Lohr

Universität Hamburg
Faculty of Mathematics, Informatics and Natural Sciences
Department Biology
Institute of Zoology
Neurophysiology
Martin-Luther-King-Platz 3
20146 Hamburg

Dr. Christine Gee

Dept. of Synaptic Physiology

Center for Molecular Neurobiology Hamburg (ZMNH)

University Medical Center Hamburg-Eppendorf (UKE)

M.Sc. Oana Constantin

Dept. of Synaptic Physiology

Center for Molecular Neurobiology Hamburg (ZMNH)

University Medical Center Hamburg-Eppendorf (UKE)

Contact

University Medical Center Hamburg-Eppendorf 
Department of Biochemistry and Molecular Cell Biology 
Martinistrasse 52
20246 Hamburg

Scientific Coordinator

Dr. Björn-Philipp Diercks
Fon: +49  (0) 40 7410 54338
E-Mail: b.diercks©uke.de

Administration

Laura Mitsching
Fon: +49  (0) 40 7410 50301
E-Mail: l.mitsching©uke.de