A02

A02: Summary

From Diercks, B.-P. et al. (2018). ORAI1, STIM1/2, and RYR1 shape subsecond Ca2+ microdomains upon T cell activation. Sci. Signal. 11, eaat0358.

Activation of T cells is closely related to the occurrence of Ca2+ microdomains, initiated by the NAADP/RyR1 axis. However, recent data suggests contribution of Ca2+ entry involving additional players during initial microdomain formation. Our project aims to unravel the interplay of the complementary players during early T cell activation with focus on Ca2+ entry channels and adaptor proteins Orai1/2, STIM1/2, as well as autocrine purinergic signaling, e.g. via P2X7 and P2X4. Accompanied development of innovative image processing algorithms will overcome uncertainties of microscopy image post-processing and analysis of high speed live-cell imaging data.

Dr. Insa Wolf

Selected publications

 

  1. Wolf IMA, Diercks BP, Gattkowski E, Czarniak F, Kempski J, Werner R, Schetelig D, Mittrücker HW, Schumacher V, von Osten M, Lodygin D, Flügel A, Fliegert R, Guse AH (2015) Frontrunners of T cell activation: Initial, localized Ca2+ signals mediated by NAADP and the type 1 ryanodine receptor. Sci Signal 8: ra102.
  2. Schetelig D, Wolf IMA, Diercks BP, Fliegert R, Guse AH, Schlaefer A, Werner R (2015) A modular framework for post-processing and analysis of fluorescence microscopy image sequences of subcellular Calcium dynamics. Proc. BVM, Springer pp. 401-406.
  3. Ernst IMA, Fliegert R, Guse AH (2013) Adenine dinucleotide second messengers and T-lymphocyte calcium signaling. Front Immunol 4:259.
  4. Ernst IMA, Kleszczynski K, Wagner AE, Rimbach G, Tobias TW (2013) Sulforaphane and phenylethyl isothiocyanate protect human skin against UVR-induced oxidative stress and apoptosis: role of Nrf2dependent gene expression and antioxidant enzymes. Pharmacol Res 78:28-40.
  5. Ernst IMA, Pallauf K, Bendall JK, Paulsen L, Nikolai S, Huebbe P, Roeder T, Rimbach G (2012) Vitamin E supplementation and lifespan in model organisms. Ageing Res Rev, 12:365-375.
  6. Philipp ER, Wessels W, Gruber H, Strahl J, Wagner AE, Ernst IMA, Rimbach G, Kraemer L, Schreiber S, Abele D, Rosenstiel P (2012) Gene expression and physiological changes of dif-ferent populations of the long-lived bivalve Arctica islandica under low oxygen conditions. PLoS One 7:e44621.
  7. Esatbeyoglu T, Huebbe P, Ernst IMA, Chin D, Wagner AE, Rimbach G (2012) Curcumin – from molecule to biological function. Angew Chem Int Ed 51:5308-5332.
  8. Ernst IMA, Wagner AE, Schuemann C, Storm N, Höppner W, Döring F, Stocker A, Rimbach G (2011) Allyl-, butyl- and phenylethyl-isothiocyanate activate Nrf2 in cultured fibroblasts. Pharmacol Res 63:233-240.
  9. Bayram B, Ozcelik B, Grimm S, Roeder T, Schrader C, Ernst IMA, Wagner AE, Grune T, Frank J, Rimbach G (2011) A diet rich in olive oil phenolics reduces oxidative stress in the heart of SAMP8 mice by induction of Nrf2-dependent gene expression. Rejuvenation Res 15:71-81.
  10. Ernst IMA, Wagner AE, Lipinski S, Skrbek S, Ruefer CE, Desel C, Rimbach G (2010) Cellular uptake, stability, visualization by ‘Naturstoff reagent A’, and multidrug resistance protein 1 gene regulatory activity of cyanidin in human keratinocytes. Pharmacol Res 61:253-258.

 

 

 

 

PD Dr. René Werner

Selected publications

 

  1. Wilms M*, Werner R*, Yamamoto T, Handels H, Ehrhardt J (2017) Subpopulation-based correspondence modeling for improved motion estimation in the presence of inter-fraction motion variations. Phys Med Biol 62:5823-5839. *equal contribution as first author
  2. Antoni S-T, Ismail ONF, Schetelig D, Diercks B-P, Werner R, Wolf IMA, Guse AH, Schlaefer A (2017) Systematic analysis of Jurkat T-cell deformation in fluorescence microscopy data. Proc BVM, Springer pp. 275-280.
  3. Wolf IMA, Diercks B-P, Gattkowski E, Czarniak F, Kempski J, Werner R, Schetelig D, Mittrücker HW, Schumacher V, von Osten M, Lodygin D, Flügel A, Fliegert R, Guse AH (2015) Frontrunners of T cell activation: Initial, localized Ca2+ signals mediated by NAADP and the type 1 ryanodine receptor. Sci Signal 8:ra120.
  4. Schetelig D, Wolf IMA, Diercks BP, Fliegert R, Guse AH, Schlaefer A, Werner R (2015) A modular framework for post-processing and analysis of fluorescence microscopy image sequences of subcellular Calcium dynamics. Proc BVM, Springer pp. 401-406.
  5. Wilms M*, Werner R*, Ehrhardt J, Schmidt-Richberg A, Schlemmer HP, Handels H (2014) Multivariate regression approaches for surrogate-based diffeomorphic estimation of respiratory motion in radiation therapy. Phys Med Biol 59:1147-1164. *equal contribution as first author
  6. Werner R, Schmidt-Richberg A, Handels H, Ehrhardt J (2014) Estimation of lung motion fields in 4D CT data by variational non-linear intensity-based registration. Phys Med Biol 59:4247-4260.
  7. Schmidt-Richberg A, Werner R, Handels H, Ehrhardt J (2012) Estimation of slipping organ motion by registration with direction-dependent regularization. Med Image Anal 16:150-159.
  8. Ehrhardt J, Werner R, Schmidt-Richberg A, Handels H (2011) Statistical modeling of 4D respiratory lung motion using diffeomorphic image registration. IEEE Trans Med Imaging 30:251-265.
  9. Werner R, Ehrhardt J, Schmidt R, Handels H (2009) Patient-specific Finite Element Modeling of respiratory lung motion using 4D CT image data. Med Phys 36:1500-1511.
  10. Ehrhardt J, Werner R, Säring D, Frenzel T, Lu W, Low DA, Handels H (2007) An optical flow based method for improved reconstruction of 4D CT data sets acquired during free breathing. Med Phys 34:711-721.

Our Team

Dr. Insa Wolf

Department of Biochemistry and Molecular Cell Biology

University Medical Center Hamburg-Eppendorf (UKE)

PD Dr. René Werner

Department of Computational Neuroscience

University Medical Center Hamburg-Eppendorf (UKE)

M.Sc. Valerie Brock

Department of Biochemistry and Molecular Cell Biology

University Medical Center Hamburg-Eppendorf (UKE)

M.Sc. Daniel Schetelig

Department of Computational Neuroscience

University Medical Center Hamburg-Eppendorf (UKE)

M.Sc. Lena Wölk

Department of Computational Neuroscience

University Medical Center Hamburg-Eppendorf (UKE)

Contact

University Medical Center Hamburg-Eppendorf 
Department of Biochemistry and Molecular Cell Biology 
Martinistrasse 52
20246 Hamburg

Scientific Coordinator

Dr. Björn-Philipp Diercks
Fon: +49  (0) 40 7410 54338
E-Mail: b.diercks©uke.de

Administration

Laura Mitsching
Fon: +49  (0) 40 7410 50301
E-Mail: l.mitsching©uke.de