A02

A02 Project movie

From Diercks, B.-P. et al. (2018). ORAI1, STIM1/2, and RYR1 shape subsecond Ca2+ microdomains upon T cell activation. Sci. Signal. 11, eaat0358.

A02:

From Diercks, B.-P. et al. (2018). ORAI1, STIM1/2, and RYR1 shape subsecond Ca2+ microdomains upon T cell activation. Sci. Signal. 11, eaat0358.

Activation of T cells is closely related to the occurrence of Ca2+ microdomains, initiated by the NAADP/RyR1 axis. However, recent data suggests contribution of Ca2+ entry involving additional players during initial microdomain formation. Our project aims to unravel the interplay of the complementary players during early T cell activation with focus on Ca2+ entry channels and adaptor proteins Orai1/2, STIM1/2, as well as autocrine purinergic signaling, e.g. via P2X7 and P2X4. Accompanied development of innovative image processing algorithms will overcome uncertainties of microscopy image post-processing and analysis of high speed live-cell imaging data.

Dr. Björn-Philipp Diercks

Selected publications (*equal contribution)

 

  1. Gu F, Krüger A, Roggenkamp HG, Alpers R, Lodygin D, Jaquet V, Möckl F, Hernandez C LC, Winterberg K, Bauche A, Rosche A, Grasberger H, Kao JY, Schetelig D, Werner R, Schröder K, Carty M, Bowie AG, Huber S, Meier C, Mittrücker HW, Heeren J, Krause KH, Flügel A, Diercks B-P*, Guse AH*. Dual NADPH oxidases DUOX1 and DUOX2 synthesize NAADP and are necessary for Ca2+ signaling during T cell activation. Science Signal. 2021; 14 eabe3800.
  2. Roggenkamp HG, Khansahib I, Hernandez C LC, Zhang Y, Lodygin D, Krüger A, Gu F, Möckl F, Löhndorf A, Wolters V, Woike D, Rosche A, Bauche A, Schetelig D, Werner R, Schlüter H, Failla AV, Meier C, Fliegert R, Walseth TF, Flügel A, Diercks B-P*, Guse AH*. HN1L/JPT2: A signaling protein that connects NAADP generation to Ca2+ microdomain formation. Science Signal. 2021; 14:eabd5647.
  3. Woelk LM, Kannabiran SA, Brock VJ, Gee CE, Lohr C, Guse AH, Diercks B-P, Werner R. Time Dependent Image Restoration of Low-SNR Live-Cell Ca2+ Fluorescence Microscopy Data. Int. J. Mol. Sci. 2021; 22, 11792.
  4. Nawrocki M, Lory N, Bedke T, Stumme F, Diercks B-P, Guse AH, Meier C, Gagliani N, Mittrücker HW, Huber S. Trans-Ned 19-Mediated Antagonism of Nicotinic Acid Adenine Nucleotide - Mediated Calcium Signaling Regulates Th17 Cell Plasticity in Mice. Cells 2021; 10, 3039.
  5. Löhndorf A, Hosang L, Dohle W, Odoardi F, Waschkowski SA, Rosche A, Bauche A, Winzer R, Tolosa E, Windhorst S, Marry S, Flügel A, Potter BVL, Diercks B-P*, Guse AH*. 2-Methoxyestradiol and its derivatives inhibit store-operated Ca2+ entry in T cells: identification of a new and potent inhibitor. BBA Mol Cell Res. 2021; 118988.
  6. Hadaeghi F, Diercks B-P, Schetelig D, Damicelli F, Wolf IMA, Werner R (2021) Spatio-temporal feature learning with reservoir computing for T-cell segmentation in live-cell Ca2+ fluorescence microscopy. Sci Rep. 2021; 11:8233.
  7. Diercks BP, Werner R, Schetelig D, Wolf IMA, Guse AH (2019) High-Resolution Calcium Imaging Method for Local Calcium Signaling. Methods Mol Biol. 1929:27-39.
  8. Diercks BP, Werner R, Weidemüller P, Czarniak F, Hernandez L, Lehmann C, Rosche A, Krüger A, Kaufmann U, Vaeth M, Failla AV, Zobiak B, Kandil FI, Schetelig D, Ruthenbeck A, Meier C, Lodygin D, Flügel A, Ren D, Wolf IMA, Feske S, Guse AH (2018) ORAI1, STIM1/2, and RYR1 shape subsecond Ca2+ microdomains upon T cell activation. Sci Signal 18;11(561).
  9. Diercks BP, Fliegert R, Guse AH (2016) Mag-Fluo4 in T Cells: Imaging of Intra-Organelle Free Ca2+Concentrations. Biochim Biophys Acta Mol Cell Res. 2017 Jun;1864(6):977-986.
  10. Wolf IMA, Diercks BP, Gattkowski E, Czarniak F, Kempski J, Werner R, Schetelig D, Mittrücker HW, Schumacher V, von Osten M, Lodygin D, Flügel A, Fliegert R, Guse AH (2015) Frontrunners of T cell activation: Initial, localized Ca2+ signals mediated by NAADP and the type 1 ryanodine receptor. Sci Signal 8: ra102.

 

 

 

 

PD Dr. René Werner

Selected publications

 

  1. Li D, Zavaglia M, Wang G, Xie H, Hu Y, Werner R, Guan JS, Hilgetag CC (2019) Discrimination of the hierarchical structure of cortical layers in 2-photon microscopy data by combined unsupervised and supervised machine learning. Sci Rep  15;9(1):7424
  2. Wilms M*, Werner R*, Yamamoto T, Handels H, Ehrhardt J (2017) Subpopulation-based correspondence modeling for improved motion estimation in the presence of inter-fraction motion variations. Phys Med Biol 62:5823-5839. *equal contribution as first author
  3. Antoni S-T, Ismail ONF, Schetelig D, Diercks B-P, Werner R, Wolf IMA, Guse AH, Schlaefer A (2017) Systematic analysis of Jurkat T-cell deformation in fluorescence microscopy data. Proc BVM, Springer pp. 275-280.
  4. Wolf IMA, Diercks B-P, Gattkowski E, Czarniak F, Kempski J, Werner R, Schetelig D, Mittrücker HW, Schumacher V, von Osten M, Lodygin D, Flügel A, Fliegert R, Guse AH (2015) Frontrunners of T cell activation: Initial, localized Ca2+ signals mediated by NAADP and the type 1 ryanodine receptor. Sci Signal 8:ra120.
  5. Schetelig D, Wolf IMA, Diercks BP, Fliegert R, Guse AH, Schlaefer A, Werner R (2015) A modular framework for post-processing and analysis of fluorescence microscopy image sequences of subcellular Calcium dynamics. Proc. BVM, Springer pp. 401-406.
  6. Wilms M*, Werner R*, Ehrhardt J, Schmidt-Richberg A, Schlemmer HP, Handels H (2014) Multivariate regression approaches for surrogate-based diffeomorphic estimation of respiratory motion in radiation therapy. Phys Med Biol59:1147-1164. *equal contribution as first author
  7. Werner R, Schmidt-Richberg A, Handels H, Ehrhardt J (2014) Estimation of lung motion fields in 4D CT data by variational non-linear intensity-based registration. Phys Med Biol 59:4247-4260.
  8. Schmidt-Richberg A, Werner R, Handels H, Ehrhardt J (2012) Estimation of slipping organ motion by registration with direction-dependent regularization. Med Image Anal 16:150-159.
  9. Ehrhardt J, Werner R, Schmidt-Richberg A, Handels H (2011) Statistical modeling of 4D respiratory lung motion using diffeomorphic image registration. IEEE Trans Med Imaging 30:251-265.
  10. Werner R, Ehrhardt J, Schmidt R, Handels H (2009) Patient-specific Finite Element Modeling of respiratory lung motion using 4D CT image data. Med Phys 36:1500-1511.

Our Team

Dr. Björn-Philipp Diercks

Department of Biochemistry and Molecular Cell Biology

University Medical Center Hamburg-Eppendorf (UKE)

PD Dr. René Werner

Department of Computational Neuroscience

University Medical Center Hamburg-Eppendorf (UKE)

M.Sc. Valerie Brock

 
Department of Biochemistry and Molecular Cell Biology

University Medical Center Hamburg-Eppendorf (UKE)

Dr. Lena Woelk

 
Department of Computational Neuroscience

University Medical Center Hamburg-Eppendorf (UKE)

Dr. Fatemeh Hadaeghi

Department of Computational Neuroscience

University Medical Center Hamburg-Eppendorf (UKE)

Alumni

PD Dr. Insa Wolf

Department of Biochemistry and Molecular Cell Biology

University Medical Center Hamburg-Eppendorf (UKE)

Dr. Daniel Schetelig

Department of Computational Neuroscience

University Medical Center Hamburg-Eppendorf (UKE)

Contact

University Medical Center Hamburg-Eppendorf 
Department of Biochemistry and Molecular Cell Biology 
Martinistrasse 52
20246 Hamburg

Scientific Coordinator

Dr. Björn-Philipp Diercks
Fon: +49  (0) 40 7410 54338
E-Mail: b.diercks©uke.de

Administration

Laura Mitsching
Fon: +49  (0) 40 7410 50301
E-Mail: l.mitsching©uke.de