A20

A20

We hypothesize that soluble factors derived from adipose tissue under inflammation, such as ATP and ADO, differentially control dendritic cell (DC) migration. In order to test this hypothesis we will: (i) analyze purinergic signaling during the migration of murine DCs, by using microfluidic devices; (ii) evaluate Ca²⁺ signaling during the DC migration, with a focus in the ER, by combining live-cell imaging and Ca²⁺ sensitive fluorescent probes; (iii) analyze the effect of AN-derived from adipose tissue over DC migration in different levels of complexity, 2D within microfluidics, 3D in collagen matrix, and in vivo in mouse models.

Prof. Dr. Pablo Saez

Selected publications

 

1. Lomakin AJ, Cattin CJ, Cuvelier D, Alraies Z, Molina M, Nader GPF, Srivastava N, Sáez PJ, Garcia-Arcos JM, Zhitnyak IY, Bhargava A, Driscoll MK, Welf ES, Fiolka R, Petrie RJ, De Silva NS, González-Granado JM, Manel N, Lennon-Duménil AM, Müller DJ, Piel M. The nucleus acts as a ruler tailoring cell responses to spatial constraints. Science 2020 Oct 16;370(6514):eaba2894.

2. Agliari E, Sáez PJ, Barra A, Piel M, Vargas P, Castellana M. A statistical inference approach to reconstruct intercellular interactions in cell migration experiments. Science Adv 2020; 6:eaay2103.
3. Barbier L, Sáez PJ, Attia R, Lennon-Dumenil AM, Lavi I, Piel M, Vargas P. Myosin II Activity Is Selectively Needed for Migration in Highly Confined Microenvironments in Mature Dendritic Cells. Front Immunol 2019, 10:747.
4. Villalobos-Labra R, Sáez PJ, Subiabre M, Silva L, Toledo F, Westermeier F, Pardo F, Farias M, Sobrevia L. Pre-pregnancy maternal obesity associates with endoplasmic reticulum stress in human umbilical vein endothelium. Biochim Biophys Acta Mol Basis Dis 2018; 1864:3195-3210.
5. Sáez PJ, Barbier L, Attia R, Thiam HR, Piel M, Vargas P. Leukocyte Migration and Deformation in Collagen Gels and Microfabricated Constrictions. Methods Mol Biol 2018, 1749:361-373.
6. Sáez PJ, Vargas P, Shoji KF, Harcha PA, Lennon-Dumenil AM, Sáez JC. ATP promotes the fast migration of dendritic cells through the activity of pannexin 1 channels and P2X7 receptors. Science Signal 2017, 10(506):eaah7107.
7. Bretou M, Sáez PJ, Sanseau D, Maurin M, Lankar D, Chabaud M, Spampanato C, Malbec O, Barbier L, Muallem S, Maiuri P, Ballabio A, Helft J, Piel M, Vargas P, Lennon-Dumenil AM. Lysosome signaling controls the migration of dendritic cells. Science Immunol 2017; 2(16):eaak9573.
8. Sáez PJ, Shoji KF, Retamal MA, Harcha PA, Ramirez G, Jiang JX, von Bernhardi R, Saez JC: ATP is required and advances cytokine-induced gap junction formation in microglia in vitro. Mediators Inflamm 2013; 2013:216402.
9. Sáez PJ, Orellana JA, Vega-Riveros N, Figueroa VA, Hernandez DE, Castro JF, Klein AD, Jiang JX, Zanlungo S, Saez JC: Disruption in connexin-based communication is associated with intracellular Ca(2)(+) signal alterations in astrocytes from Niemann-Pick type C mice. PLoS One 2013; 8:e71361.
10. Mendoza-Naranjo A, Sáez PJ, Johansson CC, Ramirez M, Mandakovic D, Pereda C, Lopez MN, Kiessling R, Saez JC, Salazar-Onfray F: Functional gap junctions facilitate melanoma antigen transfer and cross-presentation between human dendritic cells. J Immunol 2007; 178:6949-6957.

 

 

 

 

Our Team