A20

A20

We hypothesize that soluble factors derived from adipose tissue under inflammation, such as ATP and ADO, differentially control dendritic cell (DC) migration. In order to test this hypothesis we will: (i) analyze purinergic signaling during the migration of murine DCs, by using microfluidic devices; (ii) evaluate Ca²⁺ signaling during the DC migration, with a focus in the ER, by combining live-cell imaging and Ca²⁺ sensitive fluorescent probes; (iii) analyze the effect of AN-derived from adipose tissue over DC migration in different levels of complexity, 2D within microfluidics, 3D in collagen matrix, and in vivo in mouse models.

Prof. Dr. Pablo Saez

Selected publications

 

1. Liu J, Boix-Campos J, Ron JE, Kux JM, Oremek MEM, Rossi AG, Gov NS, Sáez PJ. Trade-off between branching and polarity controls decision-making during cell migration. Sci Adv. 2026 Jan 2;12(1):eads2734.
2. Palii SM, Voytovych A, Muzyka N, Chantada N, Sáez PJ, Álvarez E, Shevchuk O. Vessel-on-a-Chip to Study Vascular Endothelial Inflammation. Curr Protoc. 2025 Dec;5(12):e70281.
3. Alraies Z, Rivera CA, Delgado MG, Sanséau D, Maurin M, Amadio R, Maria Piperno G, Dunsmore G, Yatim A, Lacerda Mariano L, Kniazeva A, Calmettes V, Sáez PJ, Williart A, Popard H, Gratia M, Lamiable O, Moreau A, Fusilier Z, Crestey L, Albaud B, Legoix P, Dejean AS, Le Dorze AL, Nakano H, Cook DN, Lawrence T, Manel N, Benvenuti F, Ginhoux F, Moreau HD, P F Nader G, Piel M, Lennon-Duménil AM. Cell shape sensing licenses dendritic cells for homeostatic migration to lymph nodes. Nat Immunol. 2024 Jul;25(7):1193-1206.
4. Ron JE, Crestani M, Kux JM, Liu Jiayi, Al-Dam N, Monzo P, Gauthier NC, Sáez PJ, Gov NS. Emergent seesaw oscillations during cellular directional decision-making. Nat Physics. 2024 Mar;20:501-511.
5. Zhovmer AS, Manning A, Smith C, Nguyen A, Prince O, Sáez PJ, Ma X, Tsygankov D, Cartagena-Rivera AX, Singh NA, Singh RK, Tabdanov ED. Septins provide microenvironment sensing and cortical actomyosin partitioning in motile amoeboid T lymphocytes. Sci Adv. 2024 Jan 5;10(1):eadi1788.
6. Woo MS, Ufer F, Sonner JK, Belkacemi A, Tintelnot J, Sáez PJ, Krieg PF, Mayer C, Binkle-Ladisch L, Engler JB, Bauer S, Kursawe N, Vieira V, Mannebach S, Freichel M, Flockerzi V, Vargas P, Friese MA. Calcium channel β3 subunit regulates ATP-dependent migration of dendritic cells. Sci Adv. 2023 Sep 22;9(38):eadh1653.
7. Lomakin AJ, Cattin CJ, Cuvelier D, Alraies Z, Molina M, Nader GPF, Srivastava N, Sáez PJ, Garcia-Arcos JM, Zhitnyak IY, Bhargava A, Driscoll MK, Welf ES, Fiolka R, Petrie RJ, De Silva NS, González-Granado JM, Manel N, Lennon-Duménil AM, Müller DJ, Piel M. The nucleus acts as a ruler tailoring cell responses to spatial constraints. Science 2020 Oct 16;370(6514):eaba2894.
8. Agliari E, Sáez PJ, Barra A, Piel M, Vargas P, Castellana M. A statistical inference approach to reconstruct intercellular interactions in cell migration experiments. Science Adv 2020; 6:eaay2103.
9. Sáez PJ, Vargas P, Shoji KF, Harcha PA, Lennon-Dumenil AM, Sáez JC. ATP promotes the fast migration of dendritic cells through the activity of pannexin 1 channels and P2X7 receptors. Science Signal 2017, 10(506):eaah7107.
10. Bretou M, Sáez PJ, Sanseau D, Maurin M, Lankar D, Chabaud M, Spampanato C, Malbec O, Barbier L, Muallem S, Maiuri P, Ballabio A, Helft J, Piel M, Vargas P, Lennon-Dumenil AM. Lysosome signaling controls the migration of dendritic cells. Science Immunol 2017; 2(16):eaak9573.

Our Team

Proposed PI 3rd funding period