A04

A04

This project aims at the further development and the extension of chemical routes for the synthesis of lipophilic, bioreversibly modified and/or photo-caged derivatives of the highly polar second messengers nicotinic acid adenine dinucleotide phosphate (MASTER-NAADP), (2’-deoxy)adenosine diphosphate-5-ribose ((2d)ADPR) and cyclic nucleoside monophosphates (cNMPs). Furthermore, bioinspired membrane-linked, fluorescent and ATP-selective chemosensors for the determination of ATP concentrations at cell surfaces will be developed. Thus, this project will address the central demand of the intracellular delivery and the detection of polyphosphorylated adenosine metabolites.

Prof. Dr. Chris Meier

Selected publications

 

1. Winterberg KJ, Schwentner V, Gu F, Möckl F, Li G, Bauche A, Etzold S, Rosche A, Weiß M, Thuille N, Förster F, Woelk L, Werner R, Kovacevic D, Fehse B, Kurelic R, Nawrocki M, Huber S, Mittrücker HW, Meier C, Müller CE, Baier G, Skålhegg BS, de Deken X, Wahl-Schott C, Mair T, Siebels B, Cugota Canals R, Odoardi F, Lodygin D, Flügel A, Nikolaev VO, Diercks BP, Guse AH. Multiple signaling events are required for NAADP synthesis by DUOX2 and formation of Ca²⁺ microdomains to initiate T cell activation. Sci Signal. 2026 Jan 13;19(920):eadp4326.
2. Nikolova V, Linnemannstöns K, Bendel ME, Machado M, Ganter B, Budimir P, Vogts M, Fischer C, Ganter M, Meier C, Dobbelstein M. Membrane-Permeable 5-Fluorodeoxyuridine Triphosphate Derivatives Inhibit the Proliferation of Plasmodium falciparum. ACS Infect Dis. 2025 Nov 14;11(11):3138-3151.
3. Knaack JIH, List E, Stalling D, Sterrenberg VT, Meier C, Wagenknecht HA, Bosse JB. Live-Cell RNA Imaging via Clickable TriPPPro Nucleotide Reporters. Angew Chem Int Ed Engl. 2026 Jan 9;65(2):e16613.
4. Vogts M, Witt J, Riedner M, Meier C. Development of a HILIC-MS/MS method for simultaneous quantification of lipophilic antitumor TriPPPro-prodrugs and their polar metabolites in HT29 cell extracts. J Chromatogr B Analyt Technol Biomed Life Sci. 2025 Sep 1;1263:124673.
5. Krukenberg S, Möckl F, Weiß M, Dekiert P, Hofmann M, Gerlach F, Winterberg KJ, Kovacevic D, Khansahib I, Troost B, Hinrichs M, Granato V, Nawrocki M, Hub T, Tsvilovskyy V, Medert R, Woelk LM, Förster F, Li H, Werner R, Altfeld M, Huber S, Clarke OB, Freichel M, Diercks BP, Meier C*, Guse AH*. MASTER-NAADP: a membrane permeable precursor of the Ca²⁺mobilizing second messenger NAADP. Nat Commun. 2024 Sep 13;15(1):8008.
6. Sterrenberg VT, Stalling D, Knaack JIH, Soh TK, Bosse JB, Meier C. A TriPPPro-Nucleotide Reporter with Optimized Cell-Permeable Dyes for Metabolic Labeling of Cellular and Viral DNA in Living Cells. Angew Chem Int Ed Engl. 2023 Sep 18;62(38):e202308271. 
7. Nawrocki M, Lory N, Bedke T, Stumme F, Diercks BP, Guse AH, Meier C, Gagliani N, Mittrücker HW, Huber S. Trans-Ned 19-Mediated Antagonism of Nicotinic Acid Adenine Nucleotide-Mediated Calcium Signaling Regulates Th17 Cell Plasticity in Mice. Cells. 2021 Nov 5;10(11):3039.
8. Gu F, Krüger A, Roggenkamp HG, Alpers R, Lodygin D, Jaquet V, Möckl F, Hernandez C LC, Winterberg K, Bauche A, Rosche A, Grasberger H, Kao JY, Schetelig D, Werner R, Schröder K, Carty M, Bowie AG, Huber S, Meier C, Mittrücker HW, Heeren J, Krause KH, Flügel A, Diercks BP, Guse AH. Dual NADPH oxidases DUOX1 and DUOX2 synthesize NAADP and are necessary for Ca²⁺ signaling during T cell activation. Sci Signal. 2021 Nov 16;14(709):eabe3800. 
9. Zhao C, Weber S, Schols D, Balzarini J, Meier C (2020) Prodrugs of γ-Alkyl-Modified Nucleoside Triphosphates: Improved Inhibition of HIV Reverse Transcriptase. Angew Chem Int Ed Engl 59:22063-22071
10. Jia X, Schols D, Meier C (2020) Lipophilic Triphosphate Prodrugs of Various Nucleoside Analogues. J Med Chem 63:6991-7007.

 

 

 

 

Our Team

Proposed PI 3rd Funding Period

Alumni